QuB

[A Correspondent]

Does QuB allow for making the rate constants in a model dependent on temprature instead of just voltage and agonist concentrations? I haven't found this in any software so far, but I would be interested in trying it. Do you know anything about this? Maybe we could do something like this by changing the formula that determines the dependence of the rate constants (currently, voltage dependence). Is there any way to set a new formula for the rate constants?

[cnicolai]

Temperature dependence is not directly supported yet. We may add it to the model in the future, but you can do it with today's model... Whereas our rates are calculated

k = k0*P*exp(k1*Q),

temperature dependence is calculated

k = k0 * T * exp(k1 / T)

In other words, P=T and Q=1/T. You can emulate temperature dependence by making a rate both P-sensitive to temperature and Q-sensitive to (1/temperature). For ramp simulations, this means adding a d/a channel in the acquisition protocol, from (1/T0) to (1/T1) and assigning it (channel 2) to "Voltage." Assign the original temperature signal to "Ligand." For "Mac Rates" analysis of recorded data, you have to save the data as a text file, load it into a spreadsheet, add a column calculated as e.g. "=1/B$1" (if temperature is in column B), then open it back up in qub and assign the new column (a/d channel) to "Voltage."